AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12883
![Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]](https://resources.bio-techne.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-12883-img0009.jpg "Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]")
![Western Blot: AHSA1 Antibody (25F2.D9)Azide and BSA Free [NBP2-12883]](https://resources.bio-techne.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Western-Blot-NBP2-12883-img0010.jpg "Western Blot: AHSA1 Antibody (25F2.D9)Azide and BSA Free [NBP2-12883]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # 25F2.D9
Format
Azide and BSA Free
Concentration
1.2 mg/ml
Product Specifications
Immunogen
Recombinant Full Length Mouse Aha1 Protein
Localization
Cytoplasm , Endoplasmic Reticulum
Specificity
Detects approx 38 kDa. Can run up to 45 kDa on SDS-PAGE.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Western Blot: AHSA1 Antibody (25F2.D9)Azide and BSA Free [NBP2-12883]
Western Blot: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12883) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]](https://resources.bio-techne.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-12883-img0007.jpg "Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]")
Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]
Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).![Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]](https://resources.bio-techne.com/images/products/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-12883-img0011.jpg "Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]")
Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883]
Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (NBP2-12883) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.Applications for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:1000
Western Blot
1:1000
Application Notes
Use 1 ug/mL of AHA1 antibody was sufficient for detection of Aha1 in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
20 mM Potassium Phosphate, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AHSA1
Alternate Names
activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1
Gene Symbol
AHSA1
Additional AHSA1 Products
Product Documents for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
Product Specific Notices for AHSA1 Antibody (25F2.D9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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