AHSA1 Antibody (4H9-D7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12891
![Western Blot: AHSA1 Antibody (4H9-D7) [NBP2-12891]](https://resources.bio-techne.com/images/products/AHSA1-Antibody-4H9-D7-Western-Blot-NBP2-12891-img0003.jpg "Western Blot: AHSA1 Antibody (4H9-D7) [NBP2-12891]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4H9-D7
Concentration
1 mg/ml
Product Specifications
Immunogen
Human Aha1, his tagged
Localization
Cytoplasm , Endoplasmic Reticulum
Specificity
Detects approx 38kDa, can run up to 45kDa on SDS Page. It sees human Aha1, Mouse Aha1 and Mouse Aha2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for AHSA1 Antibody (4H9-D7)
Western Blot: AHSA1 Antibody (4H9-D7) [NBP2-12891]
Western Blot: AHSA1 Antibody (4H9-D7) [NBP2-12891] - analysis of Human HeLa cell lysates showing detection of Aha1 protein using Mouse Anti-Aha1 Monoclonal Antibody, Clone 4H9.D7 . Primary Antibody: Mouse Anti-Aha1 Monoclonal Antibody at 1:1000. (1) HeLa cell lysate (2) recombinant Aha1 cell lysate.![Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (4H9-D7) [NBP2-12891]](https://resources.bio-techne.com/images/products/AHSA1-Antibody-4H9-D7-Immunocytochemistry-Immunofluorescence-NBP2-12891-img0004.jpg "Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (4H9-D7) [NBP2-12891]")
Immunocytochemistry/ Immunofluorescence: AHSA1 Antibody (4H9-D7) [NBP2-12891]
Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (4H9-D7) [NBP2-12891] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AHA1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X.Applications for AHSA1 Antibody (4H9-D7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Application Notes
1 ug/ml of AHA1 Antibody was sufficient for detection of Aha1 in 10 ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.2), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AHSA1
Alternate Names
activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1
Gene Symbol
AHSA1
UniProt
Additional AHSA1 Products
Product Documents for AHSA1 Antibody (4H9-D7)
Product Specific Notices for AHSA1 Antibody (4H9-D7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...