AHSA1 Antibody (OTI1D2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46503

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D2

Concentration

1 mg/ml

View all AHSA1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-203 of human AHSA1 (NP_036243) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AHSA1 Antibody (OTI1D2)

Knockout Validated: AHSA1 Antibody (OTI1D2) [NBP2-46503]

Knockout Validated: AHSA1 Antibody (OTI1D2) [NBP2-46503]

Knockout Validated: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and AHSA1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with antiAHSA1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with antiHSP90AB1antibody as a loading control.(1:500)
Western Blot: AHSA1 Antibody (OTI1D2) [NBP2-46503]

Western Blot: AHSA1 Antibody (OTI1D2) [NBP2-46503]

Western Blot: AHSA1 Antibody (OTI1D2) [NBP2-46503] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AHSA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiAHSA1.(1:2000)
Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503]

Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503]

Immunohistochemistry-Paraffin: AHSA1 Antibody (OTI1D2) [NBP2-46503] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiAHSA1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)
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Applications for AHSA1 Antibody (OTI1D2)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AHSA1

Cochaperone that stimulates HSP90 ATPase activity . May affect a step in the endoplasmic reticulum to Golgi trafficking

Alternate Names

activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1

Gene Symbol

AHSA1

Additional AHSA1 Products

Product Documents for AHSA1 Antibody (OTI1D2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AHSA1 Antibody (OTI1D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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