Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71698

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI7E11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Aiolos/IKZF3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-301 of human IKZF3 (NP_036613) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

57.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free

Western Blot: Aiolos/IKZF3 Antibody (OTI7E11)Azide and BSA Free [NBP2-71698]

Western Blot: Aiolos/IKZF3 Antibody (OTI7E11)Azide and BSA Free [NBP2-71698]

Western Blot: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKZF3.
Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698]

Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698]

Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698]

Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698]

Immunohistochemistry: Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free [NBP2-71698] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aiolos/IKZF3

IKZF3 encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Long Name

DNA-binding Protein Aiolos

Alternate Names

AIO, IKZF3, ZNFN1A3

Gene Symbol

IKZF3

Additional Aiolos/IKZF3 Products

Product Documents for Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Aiolos/IKZF3 Antibody (OTI7E11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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