Aiolos/IKZF3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16938
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (97%), Mouse (90%), Rat (91%). Backed by our 100% Guarantee.
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Aiolos/IKZF3. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aiolos/IKZF3 Antibody
![Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938]](https://resources.bio-techne.com/images/products/Aiolos-IKZF3-Antibody-Western-Blot-NBP2-16938-img0002.jpg "Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938]")
Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938]
Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:2000.
Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938] -
Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKZF3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for Aiolos/IKZF3 Antibody
Application
Recommended Usage
Simple Western
Reported by internal validation
Western Blot
1:500-1:3000
Application Notes
In Simple Western internal validation: RPMI 8266 cell line 0.1 mg/mL as sample; separated by size; antibody dilution of 1:20; observed molecular weight was 35-77 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Aiolos/IKZF3
Long Name
DNA-binding Protein Aiolos
Alternate Names
AIO, IKZF3, ZNFN1A3
Gene Symbol
IKZF3
UniProt
Additional Aiolos/IKZF3 Products
Product Documents for Aiolos/IKZF3 Antibody
Product Specific Notices for Aiolos/IKZF3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.