Aiolos/IKZF3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24495
![Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]](https://resources.bio-techne.com/images/products/Aiolos-IKZF3-Antibody-Western-Blot-NBP2-24495-img0005.jpg "Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 350-400 of human IKZF3 was used as the immunogen.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: canine (88%), mouse (88%), rat (88%), equine (82%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Aiolos/IKZF3 Antibody - BSA Free
Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]
Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-IkZF3 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Aiolos/IKZF3 Antibody - BSA Free [NBP2-24495]](https://resources.bio-techne.com/images/products/Aiolos-IKZF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24495-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Aiolos/IKZF3 Antibody - BSA Free [NBP2-24495]")
Immunocytochemistry/ Immunofluorescence: Aiolos/IKZF3 Antibody - BSA Free [NBP2-24495]
Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP2-24495] - IKZF3 antibody was tested in Jurkat cells at a 1:40 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.![Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]](https://resources.bio-techne.com/images/products/Aiolos-IKZF3-Antibody-Western-Blot-NBP2-24495-img0004.jpg "Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]")
Western Blot: Aiolos/IKZF3 AntibodyBSA Free [NBP2-24495]
Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 0.25 ug/mL.Applications for Aiolos/IKZF3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
reported in scientific literature (PMID 34207079)
Western Blot
0.25 - 1.0 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aiolos/IKZF3
Long Name
DNA-binding Protein Aiolos
Alternate Names
AIO, IKZF3, ZNFN1A3
Gene Symbol
IKZF3
UniProt
Additional Aiolos/IKZF3 Products
Product Documents for Aiolos/IKZF3 Antibody - BSA Free
Product Specific Notices for Aiolos/IKZF3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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