Ajuba Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89570

Novus Biologicals, part of Bio-Techne
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NBP1-89570
NBP1-89570-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ajuba Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LDRLYAQRPAGFGCQESRHSYPPALGSPGALAGAGVGAAGPLERRGAQPGRHSVTGYGDCAVGARYQDELTALLRLTVGTGGREAGARGEPSGIEPSGLEEPPGPFVPEAARARMREPEAREDYFGTCIKCNKGIYGQSN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%). Human reactivity reported in scientific literature (PMID: 25961170).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ajuba Antibody

Western Blot: Ajuba Antibody [NBP1-89570]

Western Blot: Ajuba Antibody [NBP1-89570]

Western Blot: Ajuba Antibody [NBP1-89570] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: Ajuba Antibody [NBP1-89570]

Immunocytochemistry/ Immunofluorescence: Ajuba Antibody [NBP1-89570]

Immunocytochemistry/Immunofluorescence: Ajuba Antibody [NBP1-89570] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570]

Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570]

Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
View 4 more images

Applications for Ajuba Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ajuba

Ajuba is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Alternate Names

JUB

Entrez Gene IDs

84962 (Human)

Gene Symbol

AJUBA

UniProt

Q96IF1

Additional Ajuba Products

Product Documents for Ajuba Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ajuba Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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