AK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33160

Novus Biologicals, part of Bio-Techne
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NBP1-33160
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (94%), Mouse (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AK2 Antibodies »

Product Summary for AK2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human AK2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (82%).

Localization

Mitochondrion intermembrane space

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AK2 Antibody

Western Blot: AK2 Antibody [NBP1-33160]

Western Blot: AK2 Antibody [NBP1-33160]

Western Blot: AK2 Antibody [NBP1-33160] - Wild-type (WT) and AK2 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with AK2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: AK2 Antibody [NBP1-33160]

Immunocytochemistry/ Immunofluorescence: AK2 Antibody [NBP1-33160]

Immunocytochemistry/Immunofluorescence: AK2 Antibody [NBP1-33160] - Analysis of methanol-fixed HeLa, using Adenylate kinase 2 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160]

Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160]

Immunohistochemistry-Paraffin: AK2 Antibody [NBP1-33160] - Paraffin-embedded rat liver. Adenylate kinase 2 antibody [N1C2] diluted at 1:500.
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Applications for AK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AK2

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Adenylate kinase 2, mitochondrial

Alternate Names

ADK2, AK 2

Gene Symbol

AK2

Additional AK2 Products

Product Documents for AK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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