AK3 Antibody (OTI6G4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03154
![Western Blot: AK3 Antibody (OTI6G4) [NBP2-03154]](https://resources.bio-techne.com/images/products/AK3-Antibody-6G4-Western-Blot-NBP2-03154-img0002.jpg "Western Blot: AK3 Antibody (OTI6G4) [NBP2-03154]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI6G4
Concentration
0.39 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human AK3 (NP_057336) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AK3 Antibody (OTI6G4)
Western Blot: AK3 Antibody (OTI6G4) [NBP2-03154]
Western Blot: AK3 Antibody (6G4) [NBP2-03154] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3.
Immunohistochemistry-Paraffin: AK3 Antibody (6G4) [NBP2-03154] Staining of paraffin-embedded Human colon tissue using anti-AK3 mouse monoclonal antibody.
![Flow Cytometry: AK3 Antibody (OTI6G4) [NBP2-03154]](https://resources.bio-techne.com/images/products/AK3-Antibody-6G4-Flow-Cytometry-NBP2-03154-img0001.jpg "Flow Cytometry: AK3 Antibody (OTI6G4) [NBP2-03154]")
Flow Cytometry: AK3 Antibody (OTI6G4) [NBP2-03154]
Flow Cytometry: AK3 Antibody (6G4) [NBP2-03154] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK3 antibody, and then analyzed by flow cytometry.Applications for AK3 Antibody (OTI6G4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.39 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AK3
Long Name
GTP:AMP phosphotransferase AK3, mitochondrial
Alternate Names
Adenylate kinase 3, AK 3, AK3L1, AK6, AKL3L, EC 2.7.4.10
Entrez Gene IDs
50808 (Human)
Gene Symbol
AK3
Additional AK3 Products
Product Documents for AK3 Antibody (OTI6G4)
Product Specific Notices for AK3 Antibody (OTI6G4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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