AK3 Antibody (SJB3-36) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04261

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SJB3-36

Format

BSA Free

Concentration

1.0 mg/ml

View all AK3 Antibodies »

Product Specifications

Immunogen

Recombinant human AK3 (adenylaste kinase isozyme 3) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for AK3 Antibody (SJB3-36) - BSA Free

Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]

Western Blot: AK3 Antibody (SJB3-36)BSA Free [NBP1-04261]

Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AK1 Lane 2. : Recombinant Human AK2 Lane 3. : Recombinant Human AK3
Immunocytochemistry/ Immunofluorescence: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]

Immunocytochemistry/ Immunofluorescence: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]

Immunocytochemistry/Immunofluorescence: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]

Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) - BSA Free [NBP1-04261]

Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) [NBP1-04261] - Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.
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Applications for AK3 Antibody (SJB3-36) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AK3

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

Long Name

GTP:AMP phosphotransferase AK3, mitochondrial

Alternate Names

Adenylate kinase 3, AK 3, AK3L1, AK6, AKL3L, EC 2.7.4.10

Gene Symbol

AK3

UniProt

NP_057366 (Human)

Additional AK3 Products

Product Documents for AK3 Antibody (SJB3-36) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AK3 Antibody (SJB3-36) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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