AK3L1 Antibody (010)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90093

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 010

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AK3L1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human AK3L1 (Uniprot#: P27144-1; Ala 2-Tyr 223).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for AK3L1 Antibody (010)

Western Blot: AK3L1 Antibody (010) [NBP2-90093]

Western Blot: AK3L1 Antibody (010) [NBP2-90093]

Western Blot: AK3L1 Antibody (010) [NBP2-90093] - Lane A: MCF7 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa
Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093]

Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093]

Immunocytochemistry/Immunofluorescence: AK3L1 Antibody (010) [NBP2-90093] - Staining of Human AK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AK4 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation: AK3L1 Antibody (010) [NBP2-90093]

Immunoprecipitation: AK3L1 Antibody (010) [NBP2-90093]

Immunoprecipitation: AK3L1 Antibody (010) [NBP2-90093] - Lane A:0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-AK4 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AK4 rabbit monoclonal antibody,at 1:200 dilution . Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

Applications for AK3L1 Antibody (010)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AK3L1

AK3L1 encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.

Long Name

Adenylate kinase 4, mitochondrial

Alternate Names

AK 4, AK3, AK4

Gene Symbol

AK4

Additional AK3L1 Products

Product Documents for AK3L1 Antibody (010)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AK3L1 Antibody (010)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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