AK3L1 Antibody (9T5P1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16116

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16116-100ul
NBP3-16116-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9T5P1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AK3L1 Antibodies »

Product Summary for AK3L1 Antibody (9T5P1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AK3L1 (P27144). MASKLLRAVILGPPGSGKGTVCQRIAQNFGLQHLSSGHFLRENIKASTEVGEMAKQYIEKSLLVPDHVITRLMMSELENRRGQHWLLDGFPRTLGQAEAL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AK3L1 Antibody (9T5P1)

Western Blot: AK3L1 Antibody (9T5P1) [NBP3-16116]

Western Blot: AK3L1 Antibody (9T5P1) [NBP3-16116]

Western Blot: AK3L1 Antibody (9T5P1) [NBP3-16116] - Western blot analysis of extracts of various cell lines, using AK3L1 antibody (NBP3-16116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (9T5P1) [NBP3-16116]

Immunocytochemistry/ Immunofluorescence: AK3L1 Antibody (9T5P1) [NBP3-16116]

Immunocytochemistry/Immunofluorescence: AK3L1 Antibody (9T5P1) [NBP3-16116] - Immunofluorescence analysis of HepG2 cells using AK3L1 Rabbit mAb (NBP3-16116) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: AK3L1 Antibody (9T5P1) [NBP3-16116]

Immunohistochemistry-Paraffin: AK3L1 Antibody (9T5P1) [NBP3-16116]

Immunohistochemistry-Paraffin: AK3L1 Antibody (9T5P1) [NBP3-16116] - Immunohistochemistry of paraffin-embedded rat kidney using AK3L1 Rabbit mAb (NBP3-16116) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for AK3L1 Antibody (9T5P1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AK3L1

AK3L1 encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.

Long Name

Adenylate kinase 4, mitochondrial

Alternate Names

AK 4, AK3, AK4

Gene Symbol

AK4

Additional AK3L1 Products

Product Documents for AK3L1 Antibody (9T5P1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AK3L1 Antibody (9T5P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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