AK3L1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99983

Novus Biologicals, part of Bio-Techne
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NBP2-99983-100ul
NBP2-99983-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AK3L1 Antibodies »

Product Summary for AK3L1 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human AK4/AK3L1 (Uniprot#: P27144-1; Ala 2-Tyr 223).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for AK3L1 Antibody

Western Blot: AK3L1 Antibody [NBP2-99983]

Western Blot: AK3L1 Antibody [NBP2-99983]

Western Blot: AK3L1 Antibody [NBP2-99983] - Anti-AK3L1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Raji Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 26 kDa
Immunoprecipitation: AK3L1 Antibody [NBP2-99983]

Immunoprecipitation: AK3L1 Antibody [NBP2-99983]

Immunoprecipitation: AK3L1 Antibody [NBP2-99983] - AK3L1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-AK3L1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AK3L1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa

Applications for AK3L1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AK3L1

AK3L1 encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.

Long Name

Adenylate kinase 4, mitochondrial

Alternate Names

AK 4, AK3, AK4

Gene Symbol

AK4

Additional AK3L1 Products

Product Documents for AK3L1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AK3L1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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