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AKAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15319

Novus Biologicals, part of Bio-Techne
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NBP2-15319

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human AKAP1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (85%).

Localization

Mitochondrion outer membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKAP1 Antibody

Western Blot: AKAP1 Antibody [NBP2-15319]

Western Blot: AKAP1 Antibody [NBP2-15319]

Western Blot: AKAP1 Antibody [NBP2-15319] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE; antibody diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: AKAP1 Antibody [NBP2-15319]

Immunocytochemistry/ Immunofluorescence: AKAP1 Antibody [NBP2-15319]

Immunocytochemistry/Immunofluorescence: AKAP1 Antibody [NBP2-15319] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP2-15319]

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP2-15319]

Immunohistochemistry-Paraffin: AKAP1 Antibody [NBP2-15319] - Human colon carcinoma, using AKAP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for AKAP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AKAP1

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq]

Alternate Names

A kinase (PRKA) anchor protein 1, a kinase anchor protein 1, mitochondrial, AKAP121, AKAP149AKAP 149, AKAP84, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa, A-kinase anchor protein, 149kD, D-AKAP1, D-AKAP-1, Dual specificity A-kinase-anchoring protein 1, dual-specificity A-kinase anchoring protein 1, MGC1807, PRKA1, protein kinase A anchoring protein 1, protein kinase A1, Protein kinase A-anchoring protein 1, protein kinase anchoring protein 1, S-AKAP84, SAKAP84AKAP, Spermatid A-kinase anchor protein 84

Gene Symbol

AKAP1

UniProt

Additional AKAP1 Products

Product Documents for AKAP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AKAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.