AKAP12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31482

Novus Biologicals, part of Bio-Techne
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NBP1-31482
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP12 Antibodies »

Product Summary for AKAP12 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP12. The exact sequence is proprietary.

Localization

Cytoplasm, cell cortex, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKAP12 Antibody

Western Blot: AKAP12 Antibody [NBP1-31482]

Western Blot: AKAP12 Antibody [NBP1-31482]

Western Blot: AKAP12 Antibody [NBP1-31482] - Sample (30ug whole cell lysate) A:HeLa S3 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP1-31482]

Immunocytochemistry/ Immunofluorescence: AKAP12 Antibody [NBP1-31482]

Immunocytochemistry/Immunofluorescence: AKAP12 Antibody [NBP1-31482] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-31482]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-31482]

Immunohistochemistry-Paraffin: AKAP12 Antibody [NBP1-31482] - Paraffin-embedded serous OVCA, using antibody at 1:100 dilution.

Applications for AKAP12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AKAP12

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

A kinase (PRKA) anchor protein (gravin) 12, A kinase (PRKA) anchor protein 12, AKAP 250, AKAP250250kDa, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621, Gravin, kinase scaffold protein gravin, Myasthenia gravis autoantigen, myasthenia gravis autoantigen gravin

Gene Symbol

AKAP12

Additional AKAP12 Products

Product Documents for AKAP12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AKAP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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