AKAP12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66604

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-66604
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Rat

Applications

ELISA, Immunoaffinity Purification, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AKAP12 Antibodies »

Product Specifications

Immunogen

Sheep were immunized with a recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein (Uniprot: Q62766).

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for AKAP12 Antibody

Western Blot: AKAP12 Antibody [NBP2-66604]

Western Blot: AKAP12 Antibody [NBP2-66604]

Western Blot: AKAP12 Antibody [NBP2-66604] - Western blotting of total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The protein was transferred to a PVDF membrane, blocked with 1X TBS, 0.1% Tween 20/ 5% NFDM, probed with 5 ug/mL of pre-immune serum 5 ug of AKAP12 antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP at 1:10000, and detected with luminol.

Applications for AKAP12 Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKAP12

Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. AKAP12 or SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for AKAP12, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells.

Alternate Names

A kinase (PRKA) anchor protein (gravin) 12, A kinase (PRKA) anchor protein 12, AKAP 250, AKAP250250kDa, DKFZp686M0430, DKFZp686O0331, FLJ20945, FLJ97621, Gravin, kinase scaffold protein gravin, Myasthenia gravis autoantigen, myasthenia gravis autoantigen gravin

Gene Symbol

AKAP12

Additional AKAP12 Products

Product Documents for AKAP12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AKAP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.