AKAP4 Antibody (4C10)

Novus Biologicals, part of Bio-Techne | Catalog # H00008852-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKAP4 Antibodies »

Product Specifications

Immunogen

AKAP4 (NP_003877, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMAYSDTTMMSDDIDWLRSHRGVCKVDLYNPEGQQDQDRKVICFVDVSTLNVEDKDYKDAASSSSEGNLNLGSLEEKEIIVIKDTEKKDQSKTEGSVCLF

Specificity

AKAP4 - A kinase (PRKA) anchor protein 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AKAP4 Antibody (4C10)

Immunohistochemistry-Paraffin: AKAP4 Antibody (4C10) [H00008852-M02]

Immunohistochemistry-Paraffin: AKAP4 Antibody (4C10) [H00008852-M02]

Immunohistochemistry-Paraffin: AKAP4 Antibody (4C10) [H00008852-M02] - Analysis of monoclonal antibody to AKAP4 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.
ELISA: AKAP4 Antibody (4C10) [H00008852-M02]

ELISA: AKAP4 Antibody (4C10) [H00008852-M02]

ELISA: AKAP4 Antibody (4C10) [H00008852-M02] - Detection limit for recombinant GST tagged AKAP4 is approximately 0.1ng/ml as a capture antibody.

Applications for AKAP4 Antibody (4C10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AKAP4

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

A kinase (PRKA) anchor protein 4, AKAP-4, AKAP82AKAP 82, A-kinase anchor protein 4, A-kinase anchor protein 82 kDa, cancer/testis antigen 99, CT99, hAKAP82Fsc1, HIFSC1, Major sperm fibrous sheath protein, p82, PRKA4, protein kinase A anchoring protein 4, Protein kinase A-anchoring protein 4, testis-specific gene HI

Entrez Gene IDs

8852 (Human)

Gene Symbol

AKAP4

OMIM

300185 (Human)

UniProt

NP_003877 (Human)

Additional AKAP4 Products

Product Documents for AKAP4 Antibody (4C10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKAP4 Antibody (4C10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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