AKAP5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92794

Novus Biologicals, part of Bio-Techne
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NBP2-92794-0.02ml
NBP2-92794-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AKAP5 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3). METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKAP5 Antibody - BSA Free

Western Blot: AKAP5 AntibodyBSA Free [NBP2-92794]

Western Blot: AKAP5 AntibodyBSA Free [NBP2-92794]

Western Blot: AKAP5 Antibody [NBP2-92794] - Analysis of extracts of various cell lines, using AKAP5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry-Paraffin: AKAP5 Antibody - BSA Free [NBP2-92794]

Immunohistochemistry-Paraffin: AKAP5 Antibody - BSA Free [NBP2-92794]

Immunohistochemistry-Paraffin: AKAP5 Antibody [NBP2-92794] - Paraffin-embedded rat brain using AKAP5 .
Immunohistochemistry-Paraffin: AKAP5 Antibody - BSA Free [NBP2-92794]

Immunohistochemistry-Paraffin: AKAP5 Antibody - BSA Free [NBP2-92794]

Immunohistochemistry-Paraffin: AKAP5 Antibody [NBP2-92794] - Paraffin-embedded mouse brain using AKAP5 .

Applications for AKAP5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKAP5

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq]

Alternate Names

A kinase (PRKA) anchor protein 5, AKAP 79, AKAP7979kDa, A-kinase anchor protein 79 kDa, A-kinase anchoring protein 75/79, cAMP-dependent protein kinase regulatory subunit II high affinity bindingprotein, cAMP-dependent protein kinase regulatory subunit II high affinity-bindingprotein, H21

Gene Symbol

AKAP5

Additional AKAP5 Products

Product Documents for AKAP5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AKAP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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