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AKAP95/AKAP8 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92775

Novus Biologicals, part of Bio-Techne
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NBP2-92775-0.02ml
NBP2-92775-0.1ml

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1). HCLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYLKGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESSGEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEAESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AKAP95/AKAP8 Antibody - Azide and BSA Free

Western Blot: AKAP95/AKAP8 AntibodyAzide and BSA Free [NBP2-92775]

Western Blot: AKAP95/AKAP8 AntibodyAzide and BSA Free [NBP2-92775]

Western Blot: AKAP95/AKAP8 Antibody [NBP2-92775] - Analysis of extracts of various cell lines, using AKAP95/AKAP8 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody - Azide and BSA Free [NBP2-92775]

Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody - Azide and BSA Free [NBP2-92775]

Immunocytochemistry/Immunofluorescence: AKAP95/AKAP8 Antibody [NBP2-92775] - Analysis of U2OS cells using AKAP95/AKAP8 at dilution of 1:200. Blue: DAPI for nuclear staining.

Applications for AKAP95/AKAP8 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKAP95/AKAP8

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Alternate Names

A kinase (PRKA) anchor protein 8, AKAP-8, AKAP-95 AKAP 95, A-kinase anchor protein 8, A-kinase anchor protein 95 kDa, A-kinase anchor protein, 95kDa, DKFZp586B1222

Gene Symbol

AKAP8

Additional AKAP95/AKAP8 Products

Product Documents for AKAP95/AKAP8 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AKAP95/AKAP8 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov