AKR1B1 Antibody (109)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89882

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 109

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AKR1B1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human AKR1B1 (Uniprot#: P15121; Met1-Phe316).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for AKR1B1 Antibody (109)

Western Blot: AKR1B1 Antibody (109) [NBP2-89882]

Western Blot: AKR1B1 Antibody (109) [NBP2-89882]

Western Blot: AKR1B1 Antibody (109) [NBP2-89882] - Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa
Immunocytochemistry/ Immunofluorescence: AKR1B1 Antibody (109) [NBP2-89882]

Immunocytochemistry/ Immunofluorescence: AKR1B1 Antibody (109) [NBP2-89882]

Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody (109) [NBP2-89882] - Staining of Human AKR1B1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AKR1B1 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Immunoprecipitation: AKR1B1 Antibody (109) [NBP2-89882]

Immunoprecipitation: AKR1B1 Antibody (109) [NBP2-89882]

Immunoprecipitation: AKR1B1 Antibody (109) [NBP2-89882] - Lane A:0.5 mg Jurkat Whole Cell Lysate 0.5 uL anti-AKR1B1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AKR1B1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa

Applications for AKR1B1 Antibody (109)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKR1B1

Aldose reductase (also designated AKR1B1, ALDR1, ALR2 or AR) is member of the monomeric NADPH-dependent aldoketoreductase family. Aldose reductase, which has a molecular mass of 36 kDa, catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol. The resulting hyperosmotic stress to cells may be a cause of diabetic complications such as neuropathy, retinopathy, and cataracts. Aldose reductase is very similar to human aldehyde reductase, bovine prostaglandin F synthase and to the European common frog protein, rho-crystallin.

Alternate Names

ADR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, aldo-keto reductase family 1, member B1 (aldose reductase), ALDR1aldose reductase, ALR2, ARaldehyde reductase 1, EC 1.1.1, EC 1.1.1.21, Lii5-2 CTCL tumor antigen, low Km aldose reductase, MGC1804

Gene Symbol

AKR1B1

Additional AKR1B1 Products

Product Documents for AKR1B1 Antibody (109)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AKR1B1 Antibody (109)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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