AKR1B1 Antibody (CPTC-AKR1B1-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79797

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CPTC-AKR1B1-2

Concentration

0.2 mg/ml

View all AKR1B1 Antibodies »

Product Summary for AKR1B1 Antibody (CPTC-AKR1B1-2)

Immunogen

Recombinant full-length human AKR1B1protein (Uniprot: P15121)

Localization

Cytoplasmic

Marker

Adrenal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79931)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for AKR1B1 Antibody (CPTC-AKR1B1-2)

Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797]

Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797]

Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Western Blot Analysis of HeLa cell lysate usingAKR1B1 Antibody (CPTC-AKR1B1-2).
Immunocytochemistry/ Immunofluorescence: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797]

Immunocytochemistry/ Immunofluorescence: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797]

Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Immunofluorescence Analysis of A549 cells labeling AKR1B1 with AKR1B1 Antibody (CPTC-AKR1B1-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Blue)
Immunohistochemistry-Paraffin: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797]

Immunohistochemistry-Paraffin: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797]

Immunohistochemistry-Paraffin: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Formalin-fixed, paraffin-embedded human prostate stained with AKR1B1 Antibody (CPTC-AKR1B1-2).
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Applications for AKR1B1 Antibody (CPTC-AKR1B1-2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: AKR1B1

Aldose reductase (also designated AKR1B1, ALDR1, ALR2 or AR) is member of the monomeric NADPH-dependent aldoketoreductase family. Aldose reductase, which has a molecular mass of 36 kDa, catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol. The resulting hyperosmotic stress to cells may be a cause of diabetic complications such as neuropathy, retinopathy, and cataracts. Aldose reductase is very similar to human aldehyde reductase, bovine prostaglandin F synthase and to the European common frog protein, rho-crystallin.

Alternate Names

ADR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, aldo-keto reductase family 1, member B1 (aldose reductase), ALDR1aldose reductase, ALR2, ARaldehyde reductase 1, EC 1.1.1, EC 1.1.1.21, Lii5-2 CTCL tumor antigen, low Km aldose reductase, MGC1804

Gene Symbol

AKR1B1

UniProt

P15121 (Human)

Additional AKR1B1 Products

Product Documents for AKR1B1 Antibody (CPTC-AKR1B1-2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKR1B1 Antibody (CPTC-AKR1B1-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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