AKR1D1 Antibody (1C2)
Novus Biologicals, part of Bio-Techne | Catalog # H00006718-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1C2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
AKR1D1 (NP_005980, 227 a.a. ~ 326 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NPIWVNVSSPPLLKDALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYPFHDEY
Specificity
AKR1D1 (1C2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for AKR1D1 Antibody (1C2)
![Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03]](https://resources.bio-techne.com/images/products/AKR1D1-Antibody-1C2-Western-Blot-H00006718-M03-img0004.jpg "Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03]")
Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03]
Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03] - Analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2. Lane 1: AKR1D1 transfected lysatE (37.4 KDa). Lane 2: Non-transfected lysate.![Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03]](https://resources.bio-techne.com/images/products/AKR1D1-Antibody-1C2-Western-Blot-H00006718-M03-img0002.jpg "Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03]")
Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03]
Western Blot: AKR1D1 Antibody (1C2) [H00006718-M03] - Western blot analysis of AKR1D1 over-expressed 293 cell line. Lane 2 cotransfected with AKR1D1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00006718-M03. GAPDH (36.1 kDa) used as loading control.![ELISA: AKR1D1 Antibody (1C2) [H00006718-M03]](https://resources.bio-techne.com/images/products/AKR1D1-Antibody-1C2-ELISA-H00006718-M03-img0003.jpg "ELISA: AKR1D1 Antibody (1C2) [H00006718-M03]")
ELISA: AKR1D1 Antibody (1C2) [H00006718-M03]
ELISA: AKR1D1 Antibody (1C2) [H00006718-M03] - Detection limit for recombinant GST tagged AKR1D1 is approximately 0.3ng/ml as a capture antibody.Applications for AKR1D1 Antibody (1C2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: AKR1D1
Alternate Names
3o5bred, Aldo-keto reductase family 1 member D1,3-oxo-5-beta-steroid 4-dehydrogenase, aldo-keto reductase family 1, member D1 (delta4-3-ketosteroid-5-beta-reductase), CBAS2, Delta(4)-3-oxosteroid 5-beta-reductase, EC 1.3.1.3, SRD5B1beta polypeptide 1 (3-oxo-5 beta-steroid delta4-dehydrogenase beta 1)
Entrez Gene IDs
6718 (Human)
Gene Symbol
AKR1D1
OMIM
604741 (Human)
UniProt
Additional AKR1D1 Products
Product Documents for AKR1D1 Antibody (1C2)
Product Specific Notices for AKR1D1 Antibody (1C2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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