AKR1D1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33811

Novus Biologicals, part of Bio-Techne
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NBP2-33811
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AKR1D1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AKR1D1 Antibody

Western Blot: AKR1D1 Antibody [NBP2-33811]

Western Blot: AKR1D1 Antibody [NBP2-33811]

Western Blot: AKR1D1 Antibody [NBP2-33811] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: AKR1D1 Antibody [NBP2-33811]

Immunohistochemistry-Paraffin: AKR1D1 Antibody [NBP2-33811]

Immunohistochemistry-Paraffin: AKR1D1 Antibody [NBP2-33811] - Staining in human liver and kidney tissues using anti-AKR1D1 antibody. Corresponding AKR1D1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AKR1D1 Antibody [NBP2-33811]

Immunohistochemistry-Paraffin: AKR1D1 Antibody [NBP2-33811]

Immunohistochemistry-Paraffin: AKR1D1 Antibody [NBP2-33811] - Staining of human kidney shows low expression as expected.
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Applications for AKR1D1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKR1D1

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction.

Alternate Names

3o5bred, Aldo-keto reductase family 1 member D1,3-oxo-5-beta-steroid 4-dehydrogenase, aldo-keto reductase family 1, member D1 (delta4-3-ketosteroid-5-beta-reductase), CBAS2, Delta(4)-3-oxosteroid 5-beta-reductase, EC 1.3.1.3, SRD5B1beta polypeptide 1 (3-oxo-5 beta-steroid delta4-dehydrogenase beta 1)

Gene Symbol

AKR1D1

UniProt

P51857 (Human)

Additional AKR1D1 Products

Product Documents for AKR1D1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AKR1D1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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