Alanyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58166

Novus Biologicals, part of Bio-Techne
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NBP2-58166
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Alanyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: EFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alanyl tRNA synthetase Antibody

Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166]

Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166]

Western Blot: Alanyl tRNA synthetase Antibody [NBP2-58166] - Analysis using Anti-AARS antibody NBP2-58166 (A) shows similar pattern to independent antibody NBP1-87414 (B).
Immunocytochemistry/ Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP2-58166]

Immunocytochemistry/ Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP2-58166]

Immunocytochemistry/Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP2-58166] - Staining of human cell line U-251 MG shows localization to cytosol.

Applications for Alanyl tRNA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alanyl tRNA synthetase

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

Alternate Names

alanine tRNA ligase 1, cytoplasmic, Alanine--tRNA ligase, alanyl-tRNA synthetase, alanyl-tRNA synthetase, cytoplasmic, AlaRS, CMT2N, EC 6.1.1, EC 6.1.1.7, Renal carcinoma antigen NY-REN-42

Gene Symbol

AARS1

Additional Alanyl tRNA synthetase Products

Product Documents for Alanyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alanyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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