Alanyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12770

Novus Biologicals, part of Bio-Techne
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NBP3-12770-100ul
NBP3-12770-50ul
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alanyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse Alanyl tRNA synthetase (Uniprot#: Q8BGQ7; Met1-Asn968).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Alanyl tRNA synthetase Antibody

Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770]

Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770]

Western Blot: Alanyl tRNA synthetase Antibody [NBP3-12770] - Lane A: NIH3T3 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:107 kDaObserved band size:107 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-12770]

Immunocytochemistry/ Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-12770]

Immunocytochemistry/Immunofluorescence: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1:5000) at 4? overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770]

Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770]

Immunohistochemistry-Paraffin: Alanyl tRNA synthetase Antibody [NBP3-12770] - Staining of mouse AARS in mouse kidney with rabbit polyclonal antibody at 1:10000 dilution.
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Applications for Alanyl tRNA synthetase Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunocytochemistry/ Immunofluorescence

1:1000-1:5000

Immunohistochemistry-Paraffin

1:2500-1:15000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alanyl tRNA synthetase

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq]

Alternate Names

alanine tRNA ligase 1, cytoplasmic, Alanine--tRNA ligase, alanyl-tRNA synthetase, alanyl-tRNA synthetase, cytoplasmic, AlaRS, CMT2N, EC 6.1.1, EC 6.1.1.7, Renal carcinoma antigen NY-REN-42

Gene Symbol

AARS1

Additional Alanyl tRNA synthetase Products

Product Documents for Alanyl tRNA synthetase Antibody

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Certificate of Analysis

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Product Specific Notices for Alanyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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