Alas1 Antibody (2K1G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16487

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16487-100ul
NBP3-16487-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2K1G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alas1 Antibodies »

Product Summary for Alas1 Antibody (2K1G3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 541-640 of human ALAS1 (P13196). TEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLVTWKQVGLELKPHSSAECNFCRRPLHFEVMSEREKSYFSGLSKLVSAQA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alas1 Antibody (2K1G3)

Western Blot: Alas1 Antibody (2K1G3) [NBP3-16487]

Western Blot: Alas1 Antibody (2K1G3) [NBP3-16487]

Western Blot: Alas1 Antibody (2K1G3) [NBP3-16487] - Western blot analysis of extracts of various cell lines, using Alas1 Rabbit mAb (NBP3-16487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Applications for Alas1 Antibody (2K1G3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alas1

Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM]

Alternate Names

5-aminolevulinic acid synthase 1, ALAS, ALAS3, ALASH, ALAS-H, aminolevulinate, delta-, synthase 1, Delta-ALA synthase 1,5-aminolevulinate synthase, nonspecific, mitochondrial, Delta-aminolevulinate synthase 1, EC 2.3.1, EC 2.3.1.37, MIG4, migration-inducing protein 4

Gene Symbol

ALAS1

Additional Alas1 Products

Product Documents for Alas1 Antibody (2K1G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Alas1 Antibody (2K1G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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