ALAS2 Antibody (4D8)
Novus Biologicals, part of Bio-Techne | Catalog # H00000212-M02
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4D8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ALAS2 (NP_000023, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFK
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
ALAS2 - aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia) (4D8)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ALAS2 Antibody (4D8)
![Western Blot: ALAS2 Antibody (4D8) [H00000212-M02]](https://resources.bio-techne.com/images/products/ALAS2-Antibody-4D8-Western-Blot-H00000212-M02-img0001.jpg "Western Blot: ALAS2 Antibody (4D8) [H00000212-M02]")
Western Blot: ALAS2 Antibody (4D8) [H00000212-M02]
Western Blot: ALAS2 Antibody (4D8) [H00000212-M02] - ALAS2 monoclonal antibody (M02), clone 4D8. Western Blot analysis of ALAS2 expression in Raw 264.7 ( Cat # L024V1 ).![ELISA: ALAS2 Antibody (4D8) [H00000212-M02]](https://resources.bio-techne.com/images/products/ALAS2-Antibody-4D8-ELISA-H00000212-M02-img0002.jpg "ELISA: ALAS2 Antibody (4D8) [H00000212-M02]")
ELISA: ALAS2 Antibody (4D8) [H00000212-M02]
ELISA: ALAS2 Antibody (4D8) [H00000212-M02] - Detection limit for recombinant GST tagged ALAS2 is approximately 0.3ng/ml as a capture antibody.Applications for ALAS2 Antibody (4D8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ALAS2
Alternate Names
5-aminolevulinic acid synthase 2,5-aminolevulinate synthase, erythroid-specific, mitochondrial, ALASE, ALAS-E, aminolevulinate, delta-, synthase 2, aminolevulinate, delta-, synthase 2 (sideroblastic/hypochromic anemia), ANH1, ASBXLDPP, Delta-ALA synthase 2, delta-ALA synthetase, Delta-aminolevulinate synthase 2, EC 2.3.1.37, FLJ93603, XLSA
Entrez Gene IDs
212 (Human)
Gene Symbol
ALAS2
UniProt
Additional ALAS2 Products
Product Documents for ALAS2 Antibody (4D8)
Product Specific Notices for ALAS2 Antibody (4D8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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