ALCAM/CD166 Antibody (8E12C7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37358
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 8E12C7
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human ALCAM/CD166 (AA: 48-216) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
65.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ALCAM/CD166 Antibody (8E12C7) - BSA Free
Western Blot: ALCAM/CD166 Antibody (8E12C7)BSA Free [NBP2-37358]
Western Blot: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.Immunocytochemistry/ Immunofluorescence: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]
Immunocytochemistry/Immunofluorescence: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunofluorescence analysis of Hela cells using ALCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) - BSA Free [NBP2-37358]
Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALCAM mouse mAb with DAB staining.Applications for ALCAM/CD166 Antibody (8E12C7) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ALCAM/CD166
Long Name
Activated Leukocyte Cell Adhesion Molecule
Alternate Names
CD166
Gene Symbol
ALCAM
Additional ALCAM/CD166 Products
Product Documents for ALCAM/CD166 Antibody (8E12C7) - BSA Free
Product Specific Notices for ALCAM/CD166 Antibody (8E12C7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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