alcohol dehydrogenase 4 Antibody (3C5)

Novus Biologicals, part of Bio-Techne | Catalog # H00000127-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alcohol dehydrogenase 4 Antibodies »

Product Specifications

Immunogen

ADH4 (NP_000661, 52 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SVIDSKFEGLAFPVIVGHEAAGIVESIGPGVTNVKPGDKVIPLYAPLCRKCKFCLSPLTNLCGKISNLKSPASDQQLMEDKTSRFTCKGKPVYHFFGTS

Specificity

ADH4 - alcohol dehydrogenase 4 (class II), pi polypeptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for alcohol dehydrogenase 4 Antibody (3C5)

Western Blot: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01]

Western Blot: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01]

Western Blot: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01] - Analysis of ADH4 expression in transfected 293T cell line by ADH4 monoclonal antibody (M01), clone 3C5. Lane 1: ADH4 transfected lysate(40.2 KDa). Lane 2: Non-transfected lysate.
Western Blot: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01]

Western Blot: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01]

Western Blot: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01] - Analysis of ADH4 over-expressed 293 cell line, cotransfected with ADH4 Validated Chimera RNAi ( Cat # H00000127-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ADH4 monoclonal antibody (M01), clone 3C5 (Cat # H00000127-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01]

ELISA: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01]

ELISA: alcohol dehydrogenase 4 Antibody (3C5) [H00000127-M01] - Detection limit for recombinant GST tagged ADH4 is approximately 0.3ng/ml as a capture antibody.

Applications for alcohol dehydrogenase 4 Antibody (3C5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 4

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Alternate Names

ADH-2, alcohol dehydrogenase 4, alcohol dehydrogenase 4 (class II), pi polypeptide, Alcohol dehydrogenase class II pi chain, aldehyde reductase, EC 1.1.1, EC 1.1.1.1

Entrez Gene IDs

127 (Human)

Gene Symbol

ADH4

OMIM

103740 (Human)

UniProt

NP_000661 (Human)

Additional alcohol dehydrogenase 4 Products

Product Documents for alcohol dehydrogenase 4 Antibody (3C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alcohol dehydrogenase 4 Antibody (3C5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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