alcohol dehydrogenase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35880
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 270-370 of human alcohol dehydrogenase (NP_000659.2).
Sequence:
LLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF
Sequence:
LLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for alcohol dehydrogenase Antibody
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] -
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] - Immunofluorescence analysis of paraffin-embedded Mouse liver using alcohol dehydrogenase Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: alcohol dehydrogenase Antibody [NBP3-35880] -
Western Blot: alcohol dehydrogenase Antibody [NBP3-35880] - Western blot analysis of lysates from Mouse lung, using alcohol dehydrogenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] -
Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase Antibody [NBP3-35880] - Immunofluorescence analysis of paraffin-embedded Rat liver using alcohol dehydrogenase Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for alcohol dehydrogenase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: alcohol dehydrogenase
Alternate Names
ADH, alpha subunit, ADH1alcohol dehydrogenase 1A, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase subunit alpha, aldehyde reductase, EC 1.1.1, EC 1.1.1.1
Gene Symbol
ADH1A
Additional alcohol dehydrogenase Products
Product Documents for alcohol dehydrogenase Antibody
Product Specific Notices for alcohol dehydrogenase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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