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Aldehyde dehydrogenase 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17798

Novus Biologicals, part of Bio-Techne
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NBP3-17798-100ul
NBP3-17798-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Aldehyde dehydrogenase 5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ESIYNEFLERTVEKAKQRKVGNPFELDTQQGPQVDKEQFERVLGYIQLGQK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldehyde dehydrogenase 5 Antibody

Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP3-17798]

Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP3-17798]

Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP3-17798] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP3-17798]

Immunocytochemistry/ Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP3-17798]

Immunocytochemistry/Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP3-17798] - Staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP3-17798]

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP3-17798]

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP3-17798] - Staining of human liver shows strong cytoplasmic positivity with a granular pattern in hepatocytes.

Applications for Aldehyde dehydrogenase 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldehyde dehydrogenase 5

Aldehyde dehydrogenase 5 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]

Alternate Names

aldehyde dehydrogenase 1 family, member B1, Aldehyde dehydrogenase 5, Aldehyde dehydrogenase family 1 member B1, ALDH class 2, ALDH5aldehyde dehydrogenase X, mitochondrial, ALDHXacetaldehyde dehydrogenase 5, EC 1.2.1, EC 1.2.1.3, MGC2230, mitochondrial aldehyde dehydrogenase X

Gene Symbol

ALDH1B1

Additional Aldehyde dehydrogenase 5 Products

Product Documents for Aldehyde dehydrogenase 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldehyde dehydrogenase 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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