ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70154

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Aldehyde Dehydrogenase 1-A3/ALDH1A3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-100 and 413-512 of human ALDH1A3 (NP_000648) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

Western Blot: ALDH1A3 Antibody (OTI4E8)Azide and BSA Free [NBP2-70154]

Western Blot: ALDH1A3 Antibody (OTI4E8)Azide and BSA Free [NBP2-70154]

Western Blot: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human ALDH1A3 (NP_000648) gene (the fusion of amino acids 1-100 and 413-512) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-P
Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154]

Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154]

Immunohistochemistry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154]

Flow Cytometry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154]

Flow Cytometry: ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free [NBP2-70154] - Analysis of Jurkat cells, using ALDH1A3 antibody (Red), compared to a nonspecific negative control antibody (Blue).
View 2 more images

Applications for ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A3/ALDH1A3

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq].

Long Name

Aldehyde Dehydrogenase 1 Family Member A3

Alternate Names

ALDH1A3, ALDH6, MCOP8, RALDH3

Gene Symbol

ALDH1A3

Additional Aldehyde Dehydrogenase 1-A3/ALDH1A3 Products

Product Documents for ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ALDH1A3 Antibody (OTI4E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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