Aldo-keto Reductase 1B10/AKR1B10 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93370
Key Product Details
Species Reactivity
Human
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QSSHLEDYPFDAE corresponding to C-Terminus according to NP_064695.2.
Epitope
C-QSSHLEDYPFDAE
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Aldo-keto Reductase 1B10/AKR1B10 Antibody
![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]](https://resources.bio-techne.com/images/products/AKR1B10-Antibody-Western-Blot-NB100-93370-img0003.jpg "Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]")
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with NB100-93370 in the right panel (mock transfection in first and last lanes).![Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]](https://resources.bio-techne.com/images/products/AKR1B10-Antibody-Western-Blot-NB100-93370-img0002.jpg "Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]")
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370]
Western Blot: Aldo-keto Reductase 1B10/AKR1B10 Antibody [NB100-93370] - Analysis of AKR1B10 using this antibody at 0.03 ug/ml in A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Aldo-keto Reductase 1B10/AKR1B10 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in lysates of HEK293, A549 and HepG2 (calculated MW of 36.0 kDa band according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aldo-keto Reductase 1B10/AKR1B10
Long Name
Aldo-keto Reductase Family 1, Member B10 [Aldose Reductase]
Alternate Names
AKR1B10, AKR1B11, AKR1B12, AldoketoReductase 1B10, ARL1, HIS, HSI
Entrez Gene IDs
57016 (Human)
Gene Symbol
AKR1B10
UniProt
Additional Aldo-keto Reductase 1B10/AKR1B10 Products
Product Documents for Aldo-keto Reductase 1B10/AKR1B10 Antibody
Product Specific Notices for Aldo-keto Reductase 1B10/AKR1B10 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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