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Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26773

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-26773-100ug
NBP3-26773-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # AKR1C1/9069

Concentration

0.2 mg/ml

Product Summary for Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Immunogen

Recombinant human full-length Aldo-keto Reductase 1C1/AKR1C1 protein

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Western Blot: Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069) [NBP3-26773 ] -

Western Blot Analysis of human HEPG2 lysate usingAldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069).
Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069) [NBP3-26773 ] -

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications for Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml for 30 minutes at RT
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Aldo-keto Reductase 1C1/AKR1C1

AKR1C1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Long Name

Aldo-keto Reductase Family 1, Member C1

Alternate Names

2-ALPHA-HSD, 20-ALPHA-HSD, AKR1C1, AldoketoReductase 1C1, DDH1, HAKRC, HBAB

Gene Symbol

AKR1C1

Additional Aldo-keto Reductase 1C1/AKR1C1 Products

Product Documents for Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aldo-keto Reductase 1C1/AKR1C1 Antibody (AKR1C1/9069)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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