Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32015
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE36-28
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein. (Uniprot: P42330)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28)
Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28) [NBP3-32015]
Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28) [NBP3-32015] - Western blot analysis of Aldo-keto Reductase 1C3/AKR1C3 on different lysates with Rabbit anti-Aldo-keto Reductase 1C3/AKR1C3 antibody (NBP3-32015) at 1/1,000 dilution.Lane 1: A549 cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: Human kidney tissue lysate (40 ug/Lane)
Lane 5: Mouse kidney tissue lysate (40 ug/Lane)
Lane 6: Rat kidney tissue lysate (40 ug/Lane)
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 20 seconds; ECL;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32015) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aldo-keto Reductase 1C3/AKR1C3
Long Name
Aldo-keto Reductase Family 1, Member C3
Alternate Names
AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS
Gene Symbol
AKR1C3
Additional Aldo-keto Reductase 1C3/AKR1C3 Products
Product Documents for Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28)
Product Specific Notices for Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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