Aldolase B Antibody (OTI3C6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00750
![Immunohistochemistry-Paraffin: Aldolase B Antibody (OTI3C6) [NBP2-00750]](https://resources.bio-techne.com/images/products/Aldolase-B-Antibody-3C6-Immunohistochemistry-Paraffin-NBP2-00750-img0003.jpg "Immunohistochemistry-Paraffin: Aldolase B Antibody (OTI3C6) [NBP2-00750]")
![Western Blot: Aldolase B Antibody (OTI3C6) [NBP2-00750]](https://resources.bio-techne.com/images/products/Aldolase-B-Antibody-3C6-Western-Blot-NBP2-00750-img0002.jpg "Western Blot: Aldolase B Antibody (OTI3C6) [NBP2-00750]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3C6
Concentration
0.77 mg/ml
Product Summary for Aldolase B Antibody (OTI3C6)
Immunogen
Full length human recombinant protein of human ALDOB(NP_000026) produced in HEK293 cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aldolase B Antibody (OTI3C6)
Western Blot: Aldolase B Antibody (OTI3C6) [NBP2-00750]
Western Blot: Aldolase B Antibody (3C6) [NBP2-00750] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aldolase B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Aldolase B.
Immunohistochemistry-Paraffin: Aldolase B Antibody (3C6) [NBP2-00750] Staining of paraffin-embedded Human liver tissue using anti-Aldolase B mouse monoclonal antibody.
![Flow Cytometry: Aldolase B Antibody (OTI3C6) [NBP2-00750]](https://resources.bio-techne.com/images/products/Aldolase-B-Antibody-3C6-Flow-Cytometry-NBP2-00750-img0001.jpg "Flow Cytometry: Aldolase B Antibody (OTI3C6) [NBP2-00750]")
Flow Cytometry: Aldolase B Antibody (OTI3C6) [NBP2-00750]
Flow Cytometry: Aldolase B Antibody (3C6) [NBP2-00750] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Aldolase B antibody, and then analyzed by flow cytometry.Applications for Aldolase B Antibody (OTI3C6)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.77 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aldolase B
Alternate Names
ALDO2, aldolase B, fructose-bisphosphate, EC 4.1.2.13, fructose-bisphosphatase, fructose-bisphosphate aldolase B
Entrez Gene IDs
229 (Human)
Gene Symbol
ALDOB
Additional Aldolase B Products
Product Documents for Aldolase B Antibody (OTI3C6)
Product Specific Notices for Aldolase B Antibody (OTI3C6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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