Aldolase B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87494

Novus Biologicals, part of Bio-Techne
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NBP1-87494
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aldolase B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAA

Reactivity Notes

Reactivity reported in scientific literature (PMID: 21307348).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldolase B Antibody

Western Blot: Aldolase B Antibody [NBP1-87494]

Western Blot: Aldolase B Antibody [NBP1-87494]

Western Blot: Aldolase B Antibody [NBP1-87494] - Analysis in human kidney tissue.
Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494]

Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494]

Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494] - Staining in human kidney and tonsil tissues . Corresponding ALDOB RNA-seq data are presented for the same tissues.
Western Blot: Aldolase B Antibody [NBP1-87494]

Western Blot: Aldolase B Antibody [NBP1-87494]

Western Blot: Aldolase B Antibody [NBP1-87494] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431 Lane 5: Human liver tissue
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Applications for Aldolase B Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldolase B

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13 ) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Alternate Names

ALDO2, aldolase B, fructose-bisphosphate, EC 4.1.2.13, fructose-bisphosphatase, fructose-bisphosphate aldolase B

Gene Symbol

ALDOB

Additional Aldolase B Products

Product Documents for Aldolase B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Aldolase B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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