Aldolase B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25271

Novus Biologicals, part of Bio-Techne
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NBP3-25271
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Aldolase B Antibodies »

Product Summary for Aldolase B Antibody

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Aldolase B using the following amino acid sequence: YQKDSQGKLFRNILKEKGIVVGIK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aldolase B Antibody

Aldolase B Antibody Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP3-25271]

Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP3-25271]

Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Aldolase B Antibody Western Blot: Aldolase B Antibody [NBP3-25271]

Western Blot: Aldolase B Antibody [NBP3-25271]

Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.

Applications for Aldolase B Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldolase B

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13 ) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Alternate Names

ALDO2, aldolase B, fructose-bisphosphate, EC 4.1.2.13, fructose-bisphosphatase, fructose-bisphosphate aldolase B

Gene Symbol

ALDOB

Additional Aldolase B Products

Product Documents for Aldolase B Antibody

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Certificate of Analysis

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Product Specific Notices for Aldolase B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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