Alkaline Phosphatase/ALPL Antibody (10B1M5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15273

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15273-100ul
NBP3-15273-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10B1M5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alkaline Phosphatase/ALPL Antibodies »

Product Summary for Alkaline Phosphatase/ALPL Antibody (10B1M5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alkaline Phosphatase/ALPL (P05186). MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Alkaline Phosphatase/ALPL Antibody (10B1M5)

Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273]

Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273]

Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273] - Western blot analysis of extracts of various cell lines, using Alkaline Phosphatase/ALPL (ALPL) Rabbit mAb (NBP3-15273) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273]

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273]

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273] - Immunohistochemistry of paraffin-embedded human placenta using Alkaline Phosphatase/ALPL (ALPL) Rabbit mAb (NBP3-15273) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Alkaline Phosphatase/ALPL Antibody (10B1M5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase/ALPL

Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Gene Symbol

ALPL

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Alkaline Phosphatase/ALPL Antibody (10B1M5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Alkaline Phosphatase/ALPL Antibody (10B1M5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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