Alkaline Phosphatase, Tissue Non-Specific Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91660

Novus Biologicals, part of Bio-Techne
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NBP1-91660
NBP1-91660-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Alkaline Phosphatase, Tissue Non-Specific Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Alkaline Phosphatase, Tissue Non-Specific Antibody

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660]

Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660]

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660]

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human pancreas shows no postivity in glandular cells as expected.
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Applications for Alkaline Phosphatase, Tissue Non-Specific Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Tissue Non-Specific

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Alternate Names

Alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, APTNAP, AP-TNAP, EC 3.1.3.1, FLJ40094, FLJ93059, glycerophosphatase, HOPS, liver/bone/kidney-type alkaline phosphatase, MGC161443, tissue-nonspecific ALP, TNAP, TNSALPMGC167935

Gene Symbol

ALPL

Additional Alkaline Phosphatase, Tissue Non-Specific Products

Product Documents for Alkaline Phosphatase, Tissue Non-Specific Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alkaline Phosphatase, Tissue Non-Specific Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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