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ALKBH3 Antibody (2A5-4F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00221120-M01

Novus Biologicals, part of Bio-Techne
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H00221120-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A5-4F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DEPC-1 (AAH15155, 1 a.a. ~ 139 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW

Reactivity Notes

Human. Other species not tested.

Specificity

DEPC-1 - prostate cancer antigen-1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ALKBH3 Antibody (2A5-4F5)

Western Blot: ALKBH3 Antibody (2A5-4F5) [H00221120-M01]

Western Blot: ALKBH3 Antibody (2A5-4F5) [H00221120-M01]

Western Blot: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - DEPC-1 monoclonal antibody (M01), clone 2A5-4F5 Analysis of DEPC-1 expression in K-562.
Sandwich ELISA: ALKBH3 Antibody (2A5-4F5) [H00221120-M01] - Detection limit for recombinant GST tagged DEPC-1 is approximately 0.03ng/ml as a capture antibody.

Applications for ALKBH3 Antibody (2A5-4F5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ALKBH3

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM]

Alternate Names

ABH3MGC118793, alkB, alkylation repair homolog 3 (E. coli), Alkylated DNA repair protein alkB homolog 3, DEPC1alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, DEPC-1MGC118792, EC 1.14.11, EC 1.14.11.-, FLJ43614, MGC118790, PCA1, Prostate cancer antigen 1, prostate cancer antigen-1

Entrez Gene IDs

221120 (Human)

Gene Symbol

ALKBH3

Additional ALKBH3 Products

Product Documents for ALKBH3 Antibody (2A5-4F5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALKBH3 Antibody (2A5-4F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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