ALKBH5 Antibody (HL2061) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25357
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2061
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human ALKBH5
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ALKBH5 Antibody (HL2061) - Azide and BSA Free
Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] -
Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Knockout Validated: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] -
Knockout Validated: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Wild-type (WT) and ALKBH5 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] -
Western Blot: ALKBH5 Antibody (HL2061) - Azide and BSA Free [NBP3-25357] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (NBP3-25357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for ALKBH5 Antibody (HL2061) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ALKBH5
ALKBH5 has been implicated in various physiological and pathophysiological processes. In mice, ALKBH5 is highly expressed in the testes where loss of ALKBH5 expression leads to decreased spermatogenesis, abnormal spermatozoa and increased apoptosis (3). In glioblastoma, ALKBH5 overexpression promotes stemness and tumor progression (2). In breast cancer cells under hypoxic conditions, HIF-1 alpha and HIF-2 alpha induce the expression of ALKH5 and leads to increased demethylation and expression of Nanog thus promoting breast cancer stem cell properties (4).
References
1. Chen, X. Y., Zhang, J., & Zhu, J. S. (2019). The role of m6A RNA methylation in human cancer. Molecular Cancer. https://doi.org/10.1186/s12943-019-1033-z
2. Deng, X., Su, R., Weng, H., Huang, H., Li, Z., & Chen, J. (2018). RNA N 6 -methyladenosine modification in cancers: Current status and perspectives. Cell Research. https://doi.org/10.1038/s41422-018-0034-6
3. Meyer, K. D., & Jaffrey, S. R. (2014). The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm3785
4. Zhang, C., Samanta, D., Lu, H., Bullen, J. W., Zhang, H., Chen, I., ... Semenza, G. L. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1602883113
Long Name
RNA demethylase ALKBH5
Alternate Names
ABH5, EC 1.14.11.53, OFOXD1
Gene Symbol
ALKBH5
Additional ALKBH5 Products
Product Documents for ALKBH5 Antibody (HL2061) - Azide and BSA Free
Product Specific Notices for ALKBH5 Antibody (HL2061) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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