ALKBH5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27856
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthesized peptide derived from human ALKBH5 (Uniprot #: Q6P6C2)
Specificity
Detects endogenous levels of ALKBH5
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ALKBH5 Antibody
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP3-27856] -
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP3-27856] - Immunohistochemical analysis of paraffin-embedded human tonsil.
Western Blot: ALKBH5 Antibody [NBP3-27856] -
Western Blot: ALKBH5 Antibody [NBP3-27856] - Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4C over nightApplications for ALKBH5 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-200
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 50% glycerol, 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ALKBH5
ALKBH5 has been implicated in various physiological and pathophysiological processes. In mice, ALKBH5 is highly expressed in the testes where loss of ALKBH5 expression leads to decreased spermatogenesis, abnormal spermatozoa and increased apoptosis (3). In glioblastoma, ALKBH5 overexpression promotes stemness and tumor progression (2). In breast cancer cells under hypoxic conditions, HIF-1 alpha and HIF-2 alpha induce the expression of ALKH5 and leads to increased demethylation and expression of Nanog thus promoting breast cancer stem cell properties (4).
References
1. Chen, X. Y., Zhang, J., & Zhu, J. S. (2019). The role of m6A RNA methylation in human cancer. Molecular Cancer. https://doi.org/10.1186/s12943-019-1033-z
2. Deng, X., Su, R., Weng, H., Huang, H., Li, Z., & Chen, J. (2018). RNA N 6 -methyladenosine modification in cancers: Current status and perspectives. Cell Research. https://doi.org/10.1038/s41422-018-0034-6
3. Meyer, K. D., & Jaffrey, S. R. (2014). The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm3785
4. Zhang, C., Samanta, D., Lu, H., Bullen, J. W., Zhang, H., Chen, I., ... Semenza, G. L. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1602883113
Long Name
RNA demethylase ALKBH5
Alternate Names
ABH5, EC 1.14.11.53, OFOXD1
Gene Symbol
ALKBH5
Additional ALKBH5 Products
Product Documents for ALKBH5 Antibody
Product Specific Notices for ALKBH5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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