ALOX12B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89409
![Immunohistochemistry: ALOX12B Antibody [NBP1-89409]](https://resources.bio-techne.com/images/products/ALOX12B-Antibody-Immunohistochemistry-NBP1-89409-img0008.jpg "Immunohistochemistry: ALOX12B Antibody [NBP1-89409]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ALOX12B Antibody
![Immunocytochemistry/ Immunofluorescence: ALOX12B Antibody [NBP1-89409]](https://resources.bio-techne.com/images/products/ALOX12B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89409-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ALOX12B Antibody [NBP1-89409]")
Immunocytochemistry/ Immunofluorescence: ALOX12B Antibody [NBP1-89409]
Immunocytochemistry/Immunofluorescence: ALOX12B Antibody [NBP1-89409] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409]](https://resources.bio-techne.com/images/products/ALOX12B-Antibody-Immunohistochemistry-Paraffin-NBP1-89409-img0007.jpg "Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409]")
Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409]
Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409] - Staining of human skin shows strong cytoplasmic positivity in keratinocytes.![Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409]](https://resources.bio-techne.com/images/products/ALOX12B-Antibody-Immunohistochemistry-Paraffin-NBP1-89409-img0005.jpg "Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409]")
Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409]
Immunohistochemistry-Paraffin: ALOX12B Antibody [NBP1-89409] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Applications for ALOX12B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ALOX12B
Alternate Names
12R-LOXEC 1.13.11.-, arachidonate 12-lipoxygenase, 12R type, arachidonate 12-lipoxygenase, 12R-type, EC 1.13.11.33,12R-lipoxygenase, Epidermis-type lipoxygenase 12
Gene Symbol
ALOX12B
Additional ALOX12B Products
Product Documents for ALOX12B Antibody
Product Specific Notices for ALOX12B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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