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ALOX12B Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04581

Novus Biologicals, part of Bio-Techne
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NBP3-04581-100ul
NBP3-04581-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALOX12B (NP_001130.1). MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALOX12B Antibody - Azide and BSA Free

Western Blot: ALOX12B AntibodyAzide and BSA Free [NBP3-04581]

Western Blot: ALOX12B AntibodyAzide and BSA Free [NBP3-04581]

Western Blot: ALOX12B Antibody [NBP3-04581] - Western blot analysis of extracts of Rat testis, using ALOX12B antibody (NBP3-04581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: ALOX12B AntibodyAzide and BSA Free [NBP3-04581]

Western Blot: ALOX12B AntibodyAzide and BSA Free [NBP3-04581]

Western Blot: ALOX12B Antibody [NBP3-04581] - Western blot analysis of extracts of various cell lines, using ALOX12B antibody (NBP3-04581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for ALOX12B Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALOX12B

ALOX12B, or Arachidonate 12-lipoxygenase, 12R-type has a 701 amino acid isoform that is 80 kDa, and is involved in the transformation of arachidonic acid into 12R-hydroxyeicosatetraenoic acid. Current research is being studied on the relation between ALOX12B and a variety of diseases and disorders including epithelial ovarian cancer, meningioma, breast cancer, leukemia, tonsillitis, ichthyosis, anhidrosis, autosomal recessive congenital ichthyosis, and dermatitis. This protein interacts with LMNA, KRT5, ALOX12, ALOX15, and ALOX15B in arachidonic acid metabolism, 112-eicosatetraenoic acid derivative synthesis, and lipid and lipoprotein metabolism.

Alternate Names

12R-LOXEC 1.13.11.-, arachidonate 12-lipoxygenase, 12R type, arachidonate 12-lipoxygenase, 12R-type, EC 1.13.11.33,12R-lipoxygenase, Epidermis-type lipoxygenase 12

Gene Symbol

ALOX12B

Additional ALOX12B Products

Product Documents for ALOX12B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALOX12B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov