On the product sheet of NB100-78585, it states that Synthetic peptide contain a sequence corresponding to a region within amino acids 206 and 260 of alpha 1a Adrenergic Receptor. Is this sequence conserved for only alpha 1a Adrenergic receptor or also for the 2 other alpha 1 adrenergic receptor subtypes? It also states that the Raji cell lysates were used as the positive control. Is it an cell line with over expressed adrenergic receptor? Or it is only after transfection? Does your company provide any other form of positive controls for the alpha 1 adrenergic receptor detection?

Name: alpha-1A Adrenergic R/ADRA1A Antibody
Brand: Novus Biologicals
SKU: NB100-78585

The immunogen used to generate NB100-78585 is conserved for all 9 isoforms of ADRA1A and this antibody does not cross-react with other alpha 1 adrenergic receptor subtypes. The Raji cells used were not transfected. This blot shows endogenous expression. HL-60 cells can also be used as a positive control.

alpha-1A Adrenergic R/ADRA1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-78585

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Citations (6)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Guinea Pig (100%), Monkey (100%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha-1A Adrenergic R/ADRA1A Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha-1A Adrenergic R/ADRA1A. The exact sequence is proprietary.

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-1A Adrenergic R/ADRA1A Antibody

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot alpha-1A Adrenergic R/ADRA1A protein expression. Nitrergic neuron alpha 1-, F(5, 12)=10.51, p=.0005 protein profiles are depicted in Panels 3A; GABAergic neuron alpha 1-, F(5, 12)=5.52, p=.007 protein profiles are presented in Panels 3D. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *p<.05; **p<.01; ***p<.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668/) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunocytochemistry/Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Human heart, using alpha 1a Adrenergic Receptor antibody(10 ug/ml). Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - A. Jurkat whole cell lysate/extract. B. Raji whole cell lysate/extract. C. NCI-H929 whole cell lysate/extract. 10 % SDS-PAGE alpha 1a Adrenergic Receptor antibody dilution: 1:1000

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Sample (30 ug of whole cell lysate)A:Raji 10% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Mouse prostate. alpha 1a Adrenergic Receptor antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Rat hind brain. alpha 1a Adrenergic Receptor antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] -

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha1-), alpha2- ( alpha2-), or beta1- ( beta1-) AR protein expression. Nitrergic neuron alpha1-, F(5, 12) = 10.51, p = .0005; alpha2-, F(5, 12) = 16.50, p < .0001; & beta1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha1-, F(5, 12) = 5.52, p = .007; alpha2-, F(5, 12) = 10.47, p < .0001; & beta1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha1-AR = alpha1 adrenergic receptor; alpha2-AR = alpha2 adrenergic receptor; beta1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] -

Immunocytochemistry/ Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - alpha-1A Adrenergic R/ADRA1A antibody detects alpha-1A Adrenergic R/ADRA1A protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha-1A Adrenergic R/ADRA1A stained by alpha-1A Adrenergic R/ADRA1A antibody (NB100-78585) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 um.

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Applications for alpha-1A Adrenergic R/ADRA1A Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-1A Adrenergic R/ADRA1A

Adrenergic Receptors are members of the 7-transmembrane domain G protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified to date, including three alpha 1 adrenergic receptor subtypes (1A, 1B and 1D), three alpha 2 adrenergic receptors (2A, 2B and 2C), and three beta adrenergic receptor subtypes (1, 2 and 3). Adrenergic receptors participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression and impotence. Alpha 1 adrenergic receptor subtypes are found in numerous tissues and are involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP 70), proto-oncogene expression, and mitogenesis have been linked to alpha 1 adrenergic receptor activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha 1 adrenergic receptor, which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen and progesterone.

Long Name

Alpha-1A Adrenergic Receptor

Alternate Names

a-1A AdrenergicR, ADRA1A, ADRA1L1, alpha1A AdrenergicR, ALPHA1AAR

Entrez Gene IDs

148 (Human)

Gene Symbol

ADRA1A

UniProt

P35348

Additional alpha-1A Adrenergic R/ADRA1A Products

Product Documents for alpha-1A Adrenergic R/ADRA1A Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for alpha-1A Adrenergic R/ADRA1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

alpha-1A Adrenergic R/ADRA1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-78585

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all alpha-1A Adrenergic R/ADRA1A Antibodies »

Product Specifications

Immunogen

Localization

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for alpha-1A Adrenergic R/ADRA1A Antibody

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunocytochemistry/ Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585]

Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] -

Immunocytochemistry/ Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] -

Applications for alpha-1A Adrenergic R/ADRA1A Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: alpha-1A Adrenergic R/ADRA1A

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional alpha-1A Adrenergic R/ADRA1A Products

Product Documents for alpha-1A Adrenergic R/ADRA1A Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for alpha-1A Adrenergic R/ADRA1A Antibody