alpha 2-Macroglobulin Antibody (A2M/3623)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07710

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # A2M/3623

Concentration

0.2 mg/ml

View all alpha 2-Macroglobulin Antibodies »

Product Summary for alpha 2-Macroglobulin Antibody (A2M/3623)

Immunogen

Human recombinant alpha 2-Macroglobulin protein fragment (around aa 604-748) (exact sequence is proprietary) (Uniprot: P01023)

Localization

Secreted in plasma

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08689)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for alpha 2-Macroglobulin Antibody (A2M/3623)

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710]

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710]

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).
Protein Array: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710]

Protein Array: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710]

Protein Array: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Analysis of Protein Array containing more than 19,000 full-length human proteins using alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).
alpha 2-Macroglobulin Antibody (A2M/3623)

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] -

Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).

Applications for alpha 2-Macroglobulin Antibody (A2M/3623)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: alpha 2-Macroglobulin

Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change, the protinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.

Alternate Names

A2M, alpha 2Macroglobulin, CPAMD5

Gene Symbol

A2M

UniProt

P01023 (Human)

Additional alpha 2-Macroglobulin Products

Product Documents for alpha 2-Macroglobulin Antibody (A2M/3623)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha 2-Macroglobulin Antibody (A2M/3623)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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