Alpha Actinin 4 [p Tyr4] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23333

Novus Biologicals, part of Bio-Techne
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NBP3-23333
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alpha Actinin 4 Antibodies »

Product Specifications

Immunogen

Phospho-Alpha Actinin 4 (Tyr-4) synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminus of human alpha actinin 4. This sequence is well conserved in rat and mouse alpha actinin 4, but is not conserved in other alpha actinins. [UniProt# O43707]

Modification

p Tyr4

Specificity

The antibody detects a 100 kDa protein corresponding to the molecular mass of Alpha Actinin 4 on SDS-PAGE immunoblots of human A431 cells treated with pervanadate, but is not observed after akaline phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Alpha Actinin 4 [p Tyr4] Antibody

Alpha Actinin 4 [p Tyr4] Antibody

Western Blot: Alpha Actinin 4 [p Tyr4] Antibody [NBP3-23333] -

Western Blot: Alpha Actinin 4 [p Tyr4] Antibody [NBP3-23333] - Western blot analysis of Alpha Actinin 4 in A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1,2,5.6) or after immunoprecipitation using Alpha Actinin 4 (C-terminal region) antibody in the absence (lanes 3 & 7) or presence of pervanadate-treated A431 cell lysate (lanes 4 & 8). Some lanes of the blot were treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-Alpha Actinin 4 (C-terminal region) or anti-Alpha Actinin 4 (Tyr-4).

Applications for Alpha Actinin 4 [p Tyr4] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

Additional Alpha Actinin 4 Products

Product Documents for Alpha Actinin 4 [p Tyr4] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Alpha Actinin 4 [p Tyr4] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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