alpha Adducin Antibody (2C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00000118-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all alpha Adducin Antibodies »

Product Specifications

Immunogen

ADD1 (AAH42998, 1 a.a. ~ 662 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYSFASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQDAPLSDCTETIEGLELTEQTFSPAKSLSFRKGELVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEKSPPDQPAVPHPPPSTPIKLEEGDGCAREYLLP

Specificity

ADD1 - adducin 1 (alpha)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for alpha Adducin Antibody (2C9)

Western Blot: alpha Adducin Antibody (2C9) [H00000118-M01]

Western Blot: alpha Adducin Antibody (2C9) [H00000118-M01]

Western Blot: alpha Adducin Antibody (2C9) [H00000118-M01] - ADD1 monoclonal antibody (M01), clone 2C9 Analysis of ADD1 expression in IMR-32.
Immunohistochemistry-Paraffin: alpha Adducin Antibody (2C9) [H00000118-M01]

Immunohistochemistry-Paraffin: alpha Adducin Antibody (2C9) [H00000118-M01]

Immunohistochemistry-Paraffin: alpha Adducin Antibody (2C9) [H00000118-M01] - Analysis of monoclonal antibody to ADD1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
ELISA: alpha Adducin Antibody (2C9) [H00000118-M01]

ELISA: alpha Adducin Antibody (2C9) [H00000118-M01]

ELISA: alpha Adducin Antibody (2C9) [H00000118-M01] - Detection limit for recombinant GST tagged ADD1 is approximately 0.3ng/ml as a capture antibody.

Applications for alpha Adducin Antibody (2C9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha Adducin

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Alternate Names

ADDA, adducin 1 (alpha), alpha-adducin, erythrocyte adducin alpha subunit, Erythrocyte adducin subunit alpha, MGC3339, MGC44427

Entrez Gene IDs

118 (Human)

Gene Symbol

ADD1

OMIM

102680 (Human)

UniProt

AAH42998 (Human)

Additional alpha Adducin Products

Product Documents for alpha Adducin Antibody (2C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha Adducin Antibody (2C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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