alpha Adducin Antibody (5D4H1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52409

Novus Biologicals, part of Bio-Techne
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NBP2-52409
NBP2-52409-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D4H1

Format

BSA Free

Concentration

1 mg/ml

View all alpha Adducin Antibodies »

Product Summary for alpha Adducin Antibody (5D4H1) - BSA Free

Immunogen

Purified recombinant fragment of human alpha Adducin (AA: 1-193) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Adducin Antibody (5D4H1) - BSA Free

Western Blot: alpha Adducin Antibody (5D4H1)BSA Free [NBP2-52409]

Western Blot: alpha Adducin Antibody (5D4H1)BSA Free [NBP2-52409]

Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb
against human ADD1 (AA: 1-193)
recombinant protein. (Expected MW is 47.8 kDa)
Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) - BSA Free [NBP2-52409]

Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) - BSA Free [NBP2-52409]

Immunohistochemistry-Paraffin: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis of paraffin-embedded cervical cancer tissues using alpha Adducin mAb with DAB staining.
Western Blot: alpha Adducin Antibody (5D4H1)BSA Free [NBP2-52409]

Western Blot: alpha Adducin Antibody (5D4H1)BSA Free [NBP2-52409]

Western Blot: alpha Adducin Antibody (5D4H1) [NBP2-52409] - Analysis using ADD1 mAb
against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.
View 2 more images

Applications for alpha Adducin Antibody (5D4H1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Adducin

Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; alpha and beta, or alpha and gamma subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Alternate Names

ADDA, adducin 1 (alpha), alpha-adducin, erythrocyte adducin alpha subunit, Erythrocyte adducin subunit alpha, MGC3339, MGC44427

Gene Symbol

ADD1

Additional alpha Adducin Products

Product Documents for alpha Adducin Antibody (5D4H1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for alpha Adducin Antibody (5D4H1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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